Associate Scientist, Molecular Pathology, CGR
Company: Frederick National Laboratory
Posted on: November 17, 2022
Associate Scientist, Molecular Pathology, CGR
Job ID: req2984
Employee Type: exempt full-time
Division: Clinical Research Program
Facility: Rockville: 9615 MedCtrDr
Location: 9615 Medical Center Drive, Rockville, MD 20850 USA
The Frederick National Laboratory is a Federally Funded Research
and Development Center (FFRDC) sponsored by the National Cancer
Institute (NCI) and operated by Leidos Biomedical Research, Inc.
The lab addresses some of the most urgent and intractable problems
in the biomedical sciences in cancer and AIDS, drug development and
first-in-human clinical trials, applications of nanotechnology in
medicine, and rapid response to emerging threats of infectious
Accountability, Compassion, Collaboration, Dedication, Integrity
and Versatility; it's the FNL way.
The Cancer Genomics Research Laboratory (CGR) investigates the
contribution of germline and somatic genetic variation to cancer
susceptibility and outcomes in support of the NCI's Division of
Cancer Epidemiology and Genetics (DCEG), the world's most
comprehensive cancer epidemiology research group. CGR is located at
the NCI-Shady Grove campus in Rockville, MD and operated by Leidos
Biomedical Research, Inc. We care deeply about discovering the
genetic and environmental determinants of cancer, and new
approaches to cancer prevention, through our contributions to the
molecular, genetic, and epidemiologic research of the 70+
investigators in DCEG. Working in concert with epidemiologists,
biostatisticians, and basic research scientists in DCEG's
intramural research program, CGR provides the capacity to conduct
genome-wide discovery studies and targeted regional approaches to
identify the heritable determinants of various forms of cancer.
This includes the design and analysis of high throughput studies
using various types of "-omics" technologies such as array- and
sequence-based genome-wide association studies, studies of tumor
characteristics using integrated genomic data analysis, and
molecular epidemiologic studies based on novel assays focused on
the metabolome and microbiome.
Within CGR, the Molecular and Digital Pathology Laboratory (MDPL)
supports the generation of comprehensive data on histological and
molecular profiling of tumors and their precursors including
histology, various approaches for biomarker analysis, spatial
genomics, digital pathology, and image analysis. This laboratory
section is tightly integrated with existing CGR capabilities,
including somatic genomic analyses and bioinformatics. We are
seeking an enthusiastic and experienced Associate Scientist within
the MDPL to support all aspects of assay development for laser
microdissection and other spatial biomarker assays.
- Working closely with DCEG investigators and supporting
pathologists, design and execute pilots for laser microdissection
- LM sample preparation for RNA, DNA, and protein retrieval.
- Work with digital pathology experts within MDPL to assess
experimental results, including preliminary QC and analysis and
thoughtful summarization and presentation to collaborators.
- Create and maintain RNase-free conditions for tissue handling
of fixed and frozen biological material and LM workflow.
- Develop, optimize, and troubleshoot IHC/IF development on
automated platforms for prospective image analysis, including LM
-friendly IHC/IF immunostaining assays.
- Organize day to day tasks and monitor reagent inventory for LM
and immunostaining laboratory projects.
- Ensure proper documentation of all experimental and laboratory
processes in Laboratory Information Management System (LIMS) or
other tracking systems as required.
- Perform basic histology techniques, including embedding,
microtomy, H&E staining, and coverslipping. As necessary to
meet project timelines, support MDPL staff in microtomy and hand
- Digitize H&E and IHC/IF slides using a slide scanner.
- Maintain instruments by use of preventive maintenance
procedures in accordance with established SOPs and vendor
- Train new staff on established SOPs and develop SOPs for new
- Collaborate with CGR and MDPL management and technical staff in
all aspects of assay development requiring histopathology
expertise, including spatial genomics and single-cell efforts.
To be considered for this position, you must minimally meet the
knowledge, skills, and abilities listed below:
- Possession of a Bachelor's degree from an accredited college or
university (according to CHEA) in a field related to
biomedical/biochemical research. Foreign degrees will be evaluated
for U.S. equivalency.
- A minimum of eight (8) years related biomedical/biochemical
- Knowledge of tissue laser microdissection principles and laser
- Extensive experience with LM sample preparation and LM
workflows for RNA, DNA and protein retrieval.
- Extensive experience in chromogenic and fluorescent assay
development/optimization using human FFPE and frozen tissue
sections. Demonstrated ability to troubleshoot immunostaining
assays with minimal supervision.
- Proficiency with automated histopathology instrumentation,
including tissue processors, microtomes, H&E stainers, slide
coverslippers, brightfield and fluorescent microscopes,
stereoscopes, and IHC stainers.
- Knowledge of basic histological techniques, including tissue
processing and embedding, microtomy/cryotomy, H&E staining,
slide coverslipping, microscopic recognition of major human tissue
types, and QC of unstained and stained slides.
- Careful attention to detail. Must be analytical, flexible,
innovative, and self-motivated.
- Team-oriented with excellent written and verbal communication
skills, organizational skills, and attention to detail; ability to
organize and execute multiple projects in parallel both
independently and as part of working groups, demonstrated ability
to interact effectively with cross-functional teams
- Ability to obtain and maintain a security clearance
Candidates with these desired skills will be given preferential
- HT/HTL (ASCP) certification by the American Society for
- Experience with RNA and DNA extraction from LM samples.
- Experience with IHC/IF assays on archival human FFPE oncology
- Familiarity with image analysis platforms, including Indica
Labs HALO suite.
- Experience with ISH and IHC/IF multiplex assays (RNAScope,
Ultivue and GeoMx DSP).
- Working experience with hand macro-dissection of FFPE and
frozen samples, TMA construction on automated tissue arrayer.
- Experience with brightfield and florescence slide scanners
(Leica AT2 and Zeiss Axio Scan.Z1).
- Experience with LabVantage LIMS.
- Experience with and demonstrated knowledge of proper storage,
management, and handling of a diverse collection of FFPE and frozen
- Proven experience in new technology development and
implementation of new assays into a production environment.
- Strong sense of personal responsibility and ability to work
with a team.
- Ability to prioritize, organize, and execute multiple
- This position is subject to working with or have potential for
exposure to infectious
material, requiring medical clearance and immunizations.
- This position may require continuous or intermittent exposure
to and exertion in
environmental conditions such as working outdoors, temperature
extremes, humidity extremes.
etc. as part of the job.
Equal Opportunity Employer (EOE) - Minority/Female/Disabled/Veteran
(M/F/D/V) - Drug Free Workplace (DFW)
Keywords: Frederick National Laboratory, Rockville , Associate Scientist, Molecular Pathology, CGR, Healthcare , Rockville, Maryland
Didn't find what you're looking for? Search again!